In this presented study, we established a method for diagnosis of porcine epidemic diarrhea(PED) by in situ hybridization(ISH), which made distinct progress in diagnostic pathology. We also carried out the retrospective diagnosis through ISH to assume the exact time of the first outbreak and incidence of PED in Korea.
The outbreak of PED in Korea reported in 1992. However, since the end of 1980´s, some researches of pig-industry have already suspected the outbreak of PED, not transmissible gastroenteritis(TGE). In this experiment, we performed the ISH using 80 formalin-fixed and paraffin-embedded tissues of porcine intestine which were requests for pathological diagnosis from 63 farms whose primary sign was diarrhea from 1984 to 1991.
We prepared biotinylated cDNA probe(492base pairs) for ISH by nick translation method and carried out the ISH, using Microprobe^(TM) capillary action system(Fisher Biotech^R).
We detected PED virus in intestinal mucosa of 2 cases in 1992, 1 case in 1988, and 1 case in 1987.
As a result, we assume that the outbreak of PED in Korea have already started since 1987.
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